Human CD200 PicoKine® Quick ELISA Kit

Quick ELISA Kits

CST3

OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 89 | 252 | 897 | 75 | 251 | 9606
Standard deviation | 4.36 | 16.12 | 69.06 | 4.12 | 14.30 | 72
CV (%) | 4.9% | 6.4% | 7.7% | 5.5% | 5.7% | 7.5%

monoclonal antibody from mouse|polyclonal antibody from goat

10 ng/tube

Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional)
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.