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Human CD200 PicoKine® Quick ELISA Kit

Human CD200 PicoKine® Quick ELISA Kit

Item no.	BOS-FEK0993
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Neuroscience Molecular Targets for Neuroscience Aging & Neurological Disorders ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD200/OX2\|antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2
Available
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Gene Name	CST3
Manufacturer - Specificity	Natural and recombinant Human CD200
Background	OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q31-G232
Manufacturer - Research Category	Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
Description	Human CD200 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD200 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 O.D. \| 0.015 \| 0.062 \| 0.099 \| 0.185 \| 0.329 \| 0.625 \| 1.179 \| 2.046
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 89 \| 252 \| 897 \| 75 \| 251 \| 9606 Standard deviation \| 4.36 \| 16.12 \| 69.06 \| 4.12 \| 14.30 \| 72 CV (%) \| 4.9% \| 6.4% \| 7.7% \| 5.5% \| 5.7% \| 7.5%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 89 \| 75 \| 93 \| 98 \| 88 \| 4.66 \| 5.3% Sample 2 \| 252 \| 245 \| 216 \| 254 \| 241 \| 7.47 \| 3.1% Sample 3 \| 897 \| 862 \| 897 \| 871 \| 881 \| 22.02 \| 2.5% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity\|Volume Pre-coated 96-well Strip Microplate\|1\|12 strips of 8 wells Standard\|\|2 HRP-Linked Antibody\|1\|6ml Sample Diluent\|1\|15ml TBS-T Wash Buffer (25x)\|1\|12ml Color Developing Reagent (TMB)\|1\|10ml Stop Solution\|1\|10ml Adhesive Plate Sealers\|2\|Piece
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-FEK0993-datasheet.pdf

BOS-FEK0993-safety-datasheet.pdf