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ELISA Kit for Anti-Mullerian Hormone (AMH)

ELISA Kit for Anti-Mullerian Hormone (AMH)

ArtNr	CEA228Ra-10x96T
Hersteller	Cloud-Clone
Menge	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Food Safety Hormones ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Uterine, Ovarian & Cervical Cancer
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 25.9pg/mL
Citations	Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and … Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency Long-term amelioration of an early-onset familial atrial fibrillation model with AAV-mediated in vivo gene therapy
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MIF,MIH,MIS,Müllerian Inhibiting Factor,Müllerian Inhibiting Hormone,Müllerian Inhibiting Substance
Lieferbar
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH). No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Anti-Mullerian Hormone (AMH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Anti-Mullerian Hormone (AMH) and unlabeled Anti-Mullerian Hormone (AMH) (Standards or samples) with the pre-coated antibody specific to Anti-Mullerian Hormone (AMH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample.
Manufacturer - Research Area	Endocrinology; Reproductive science; Hormone metabolism;

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