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ELISA Kit for Glutathione (GSH)

ELISA Kit for Glutathione (GSH)

ArtNr	CEA294Ge-96T
Hersteller	Cloud-Clone
Menge	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Targets for Rodent Neurosciences ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Renal Cancer Hepatic Cancer Neuronal Cell Markers
Typ	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 0.52ug/mL
Citations	Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidantsGlutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular CarcinomaAlteration in the intrafollicular thiol–redox system in infertile women with endometriosisEffects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitationOxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird's DietPrenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stressHydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathwayRapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone inductionToxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cellsHepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cellsMetabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic miceCirculating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma …Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayEpigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathwayEffects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayEffects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsProtective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formationPorous Se@ SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species …MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injuryGerminated-soy milk as a healthy diet to induce high antioxidant enzymes in breast milkHydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the HippocampusDexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative StressExosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsyAssociation Between Serum Selenium Level and Subclinical Mastitis in Dairy CattleFormaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effectNaringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic ratsMicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinomaOxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspectiveLycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise ratsκ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB PathwayAlamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl ratsIntake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseaseAntioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MiceThe κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	L-Reduced Glutathione
Lieferbar
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Glutathione (GSH). No significant cross-reactivity or interference between Glutathione (GSH) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione (GSH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione (GSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glutathione (GSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glutathione (GSH) and unlabeled Glutathione (GSH) (Standards or samples) with the pre-coated antibody specific to Glutathione (GSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glutathione (GSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glutathione (GSH) in the sample.
Manufacturer - Research Area	Metabolic pathway;

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