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ELISA Kit for Adenosine Triphosphate (ATP)

ELISA Kit for Adenosine Triphosphate (ATP)

ArtNr	CEA349Ge-48T
Hersteller	Cloud-Clone
Menge	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 4.33ng/mL
Citations	Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats Neuroprotective effects of vildagliptin in rat rotenone Parkinson' s disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways Metabolic Features of Heart Failure with Different Etiology Rifampicin-induced injury in L02 cells is alleviated by 4-PBA via inhibition of the PERK-ATF4-CHOP pathway. Rifampicin-induced injury in HepG2 cells is alleviated by TUDCA via increasing bile acid transporters expression and enhancing the Nrf2-mediated adaptive response. The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway Antihypoxic and anti-ischemic properties of the North Caucasus flora plant extracts Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling … Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat Prostaglandin E receptor subtype 4 protects against diabetic cardiomyopathy by modulating cardiac fatty acid metabolism via FOXO1/CD36 signalling Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals ¡ Estrogen inhibits bladder overactivity in rats with cyclophosphamide‐induced cystitis via downregulating the expression of P2X3 receptors in bladder epithelium cells A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Adenosine-5'-Triphosphate
Lieferbar
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Adenosine Triphosphate (ATP). No significant cross-reactivity or interference between Adenosine Triphosphate (ATP) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine Triphosphate (ATP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine Triphosphate (ATP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenosine Triphosphate (ATP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenosine Triphosphate (ATP) and unlabeled Adenosine Triphosphate (ATP) (Standards or samples) with the pre-coated antibody specific to Adenosine Triphosphate (ATP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenosine Triphosphate (ATP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenosine Triphosphate (ATP) in the sample.

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