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ELISA Kit for Thyroxine (T4)

ELISA Kit for Thyroxine (T4)

ArtNr	CEA452Ge-10x96T
Hersteller	Cloud-Clone
Menge	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Thyroid Cancer Neuronal Cell Markers
Typ	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 1.29ng/mL
Citations	Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarum Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Mice Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead 四溴双酚A对菲律宾蛤仔的生长抑制效应机理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. Stereoselective degradation and thyroid endocrine disruption of lambda-cyhalothrin in lizards (Eremias argus) following oral exposure Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring Metabolism of diflubenzuron in lizard (Eremias argus) and comparative toxicity of diflubenzuron and its metabolite Elastographic and morphological testicular changes in hypothyroidism–an experimental study Dose-dependent effects of flufenoxuron on thyroid system of mature female lizards (Eremias argus) and their offspring Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing … Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryos The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice Effect of triadimefon and its metabolite on adult amphibians Xenopus laevis Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio) Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlor Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa … Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio) Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio) Fluorene-9-Bisphenol Induced Thyroid-Disrupting Effects and Histopathological Alterations in Adult Zebrafish (Danio Rerio)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	3,5,3',5'-Tetraiodothyronine,L-Thyroxine,Levothyroxine
Lieferbar
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Thyroxine (T4). No significant cross-reactivity or interference between Thyroxine (T4) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroxine (T4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thyroxine (T4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyroxine (T4) and unlabeled Thyroxine (T4) (Standards or samples) with the pre-coated antibody specific to Thyroxine (T4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroxine (T4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyroxine (T4) in the sample.
Manufacturer - Research Area	Endocrinology; Hormone metabolism;

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