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ELISA Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI)

ELISA Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI)

ArtNr	CEA665Mu-96T
Hersteller	Cloud-Clone
Menge	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Metabolism Cholesterol & Lipid Metabolism Cell Biology ELISA Cell Stainings ECM ELISA & Immunoassays ELISA Kits Immunoassay By Organ Testicular & Prostate Cancer Lymphatic System Cancer
Typ	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 44.7pg/mL
Citations	Atp6v0d2 Is an Essential Component of the Osteoclast-Specific Proton Pump That Mediates Extracellular Acidification in Bone ResorptionBorage and fish oils lifelong supplementation decreases inflammation and improves bone health in a murine model of senile osteoporosisTRAF family member-associated NF-κB activator (TANK) is a negative regulator of osteoclastogenesis and bone formationOxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat GainXPO1/CRM1-Selective Inhibitors of Nuclear Export(SINE) reduce tumor spreading and improveoverall survival in preclinical models of prostatecancer (PCa)5-azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survivalMutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast FunctionOxytocin reverses osteoporosis in a sex-dependent mannerLanthanum Chloride Attenuates Osteoclast Formation and Function Via the DownreCavia (Guinea pig )lation of Rankl-Induced Nf-κb and Nfatc1 ActivitiesN-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities.Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia miceA novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice.Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis ResearchTSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized miceDual PI3 K/mTOR inhibition reduces prostate cancer bone engraftment altering tumor-induced bone remodelingTranscriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exerciseSignificance of the Tks4 scaffold protein in bone tissue homeostasisOsteocyte TSC1 promotes sclerostin secretion to restrain osteogenesis in miceBMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodelingEDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for OsteoporosisRNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin SynthesisA Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype …Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous …4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized MiceLgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathwayAndrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesisDefects in a liver-bone axis contribute to hepatic osteodystrophy disease progressionBMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axisFoxf2 represses bone formation via Wnt2b/β-catenin signalingExercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CTX-I,CTX1;
Lieferbar
Specificity	Mus musculus (Mouse)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type I Collagen (CTXI). No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type I Collagen (CTXI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and unlabeled Cross Linked C-Telopeptide Of Type I Collagen (CTXI) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type I Collagen (CTXI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in the sample.
Manufacturer - Research Area	Metabolic pathway; Bone metabolism;

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