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ELISA Kit for 5-Hydroxytryptamine (5-HT)

ELISA Kit for 5-Hydroxytryptamine (5-HT)

ArtNr	CEA808Ge-48T
Hersteller	Cloud-Clone
Menge	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Neuroscience Molecular Targets for Neuroscience ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 4.05ng/mL
Citations	Ginsenoside rich fraction of Panax ginseng C.A. Meyer improve feeding behavior following radiation-induced pica in ratsSensitization induces hypersensitivity in trigeminal nerveSea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsCatha edulis chewing effects on treatment of paranoid schizophrenic patientsElectroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depressionUnveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPEMetabolic Features of Heart Failure with Different Etiology Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of MigrainePI3K/AKT signaling pathway activation in a rat model of migraine.The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile AcidsChanges in sexual performance and biochemical characterisation of functional neural regions: A study in serotonin transporter knockout male ratsSerotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic InfarctionTLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway InflammationNeurotransmitter system aberrations in patients with drug addiction重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndromeWater-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stressInvestigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball playersCorrelation between intestinal microbiotal imbalance and 5‐HT metabolism, immune inflammation in chronic unpredictable mild stress male rats
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	5HT,Serotonin,N-Methyl-Gamma
Lieferbar
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum and plasma
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine (5-HT). No significant cross-reactivity or interference between 5-Hydroxytryptamine (5-HT) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptamine (5-HT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptamine (5-HT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxytryptamine (5-HT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptamine (5-HT) and unlabeled 5-Hydroxytryptamine (5-HT) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptamine (5-HT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample.
Manufacturer - Research Area	Signal transduction; Infection immunity; Neuro science; Gastroenterology;

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