ELISA Kit for Dopamine (DA)

12.35-1, 000pg/mL

The minimum detectable dose of this kit is typically less than 4.71pg/mL

2h

This assay has high sensitivity and excellent specificity for detection of Dopamine (DA).
No significant cross-reactivity or interference between Dopamine (DA) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dopamine (DA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dopamine (DA) and unlabeled Dopamine (DA) (Standards or samples) with the pre-coated antibody specific to Dopamine (DA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dopamine (DA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dopamine (DA) in the sample.

Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients; Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations; Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations; Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults; Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activity; Catha edulis chewing effects on treatment of paranoid schizophrenic patients; Neuroprotective effects of vildagliptin in rat rotenone Parkinson' s disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways; Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair test; Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in rats; Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes.; Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats; Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats; Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats; High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in rats; Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor …; Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes; Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …; Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats; Dopamine levels in human tear fluid; Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles; Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brain; Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome;