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ELISA Kit for Melatonin (MT)

ELISA Kit for Melatonin (MT)

ArtNr	CEA908Ge-96T
Hersteller	Cloud-Clone
Menge	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Food Safety Drugs Hormones Neuroscience Metabolism Glucose Homeostasis Molecular Targets for Neuroscience ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Testicular & Prostate Cancer Bone Cancer Uterine, Ovarian & Cervical Cancer
Typ	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 4.53pg/mL
Citations	Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical studyBiomarkers inducing changes due to microwave exposure effect on rat brainComparison of the flow characteristics for the honeycomb and labyrinth seal using numerical simulationThe therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave fieldEffects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in ratsImprovement of oxidative stress and immunity by melatonin: An age dependent study in golden hamsterMelatonin and its correlation with testosterone in polycystic ovarian syndromeDaily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra &hellipSerum Melatonin Level Disturbance is Related to Metabolic Syndrome and Subclinical Arterial Dysfunction in Shift Working Healthy MenMelatonin Administration Aids Bone Healing in Diabetic RatsPhotoperiodic induced melatonin regulates immunity and expression pattern of melatonin receptor MT1 in spleen and bone marrow mononuclear cells of male golden hamster.Influence of?circadian?time?and lighting conditions on expression of melatonin?receptors?1?and?2?in?murinelymphocytes.Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system Localization and expression of androgen receptor (AR) and melatonin membrane receptor (MT1R) in accessory sex organs of adult male golden hamster, Mesocricetus auratus: Modulation by photoperiod.Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brainMT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamsterPhotoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratusMelatonin prevents dexamethasone-induced testicular oxidative stress and germ cell apoptosis in golden hamster, Mesocricetus auratusReproductive seasonality in the Indian pygmy field mouse, Mus terricolorDevelopmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticusMelatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicksEffect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifersEffect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickensEffect of Monochromic Light-emitting Diode Light with Different Color on the Growth and ReproductivePerformances of Breeder Geese.Desempenho e comportamento de suínos em fase de terminação submetidos a diferentes programas de luzGreen light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain.EFFECTS OF DIURNAL AND NOCTURNAL STRENUOUS EXERCISE ON SERUM MELATONIN LEVELSRole of monochromatic light on daily variation of clock gene expression in the pineal gland ofchick.Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis inrats.单色绿光对鸡胚褪黑激素合成的影响Melatonin Supplementation Lowers OxidativeStress and Regulates Adipokines in Obese Patients on a Calorie-Restricted DietInhibitory Effect of Melatonin on Formation of the Chicken Primordial Follicles.Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicksA Circadian Rhythm in both Complement Cascade (ComC) Activation and Sphingosine-1-Phosphate (S1P) Levels in Human Peripheral Blood Supports a …Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick PinealocytesExpression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheepOCCUPATIONAL HYGIENE–PERFORMANCE COFACTOR IN POWER ENGINEERING. BIOCHEMICAL RESPONSE TO EXPOSURE TO EXTREMELY LOW …A Comparative Analysis of the Metabolic and Coagulative Profiles in Patients with Idiopathic Scoliosis, Congenital Scoliosis and Healthy Controls: A Case …cBmal1 but not cClock is associated with monochromatic green light-induced circadian rhythm of melatonin in chick pinealocytesProfile of melatonin and its receptors and synthesizing enzymes in cumulus–oocyte complexes of the developing sheep antral follicle—a potential estradiol …Effect of pinealectomy on the circadian clock of the chick retina under different monochromatic lightsPlasma levels of melatonin in dilated cardiomyopathyElevated Serum Melatonin under Constant Darkness Enhances Neural Repair in Spinal Cord Injury through Regulation of Circadian Clock Proteins ExpressionMelatonergic agonist regulates circadian clock genes and peripheral inflammatory and neuroplasticity markers in patients with depression and anxietyMel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling PathwayRole of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in miceEffect of the melatonin nuclear receptor RORα on monochromatic light-induced T-lymphocyte proliferation in chicken thymusEffect of melatonin on monochromatic light-induced changes in clock gene circadian expression in the chick liverMelatonin maintains mitochondrial membrane potential and decreases excessive intracellular Ca2+ levels in immature human oocytesConditional Controlled Light/Dark Cycle Influences Exercise-Induced Benefits in a Rat Model with Osteoarthritis: In Vitro and In Vivo StudyEffect of monochromatic light on the circadian clock of cultured chick retinal tissueSaliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexiaRole of melatonin in intestinal mucosal injury induced by restraint stress in miceHypoxia-mediated inhibition of cholesterol synthesis leads to disruption of nocturnal sex steroidogenesis in the gonad of koi carp, Cyprinus carpioSleep deprivation aggravated lipopolysaccharide/d-galactosamine-induced acute liver injury by suppressing melatonin productionEffects of tryptophan, a precursor for melatonin, on IVF outcomes and Doppler parameters急性睡眠剥夺对小鼠血浆褪黑激素水平和下丘脑钟基因表达的影响year=2020volume=5issue=1spage=46epage=52aulast=Vishwas">Influence of phytomelatonin on immune cell function in male golden hamstersInvestigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball playersMelatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in RatsA Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting ¡Identification of Serotonin as a Predictive Marker for Breast Cancer PatientsPhysiological crosstalk between the Mel1a and Mel1c pathways modulates melatonin-mediated, monochromatic light combination-induced bursa B-lymphocyte?¡MiR-375-3p mediates reduced pineal function in hypoxia-ischemia brain damageParameters of Oxidative Stress, Vitamin D, Osteopontin, and Melatonin in Patients with Lip, Oral Cavity, and Pharyngeal CancerMelatonin Nuclear Receptors Mediate Green-and-Blue-Monochromatic-Light-Combinations-Inhibited B Lymphocyte Apoptosis in the Bursa of Chickens via …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	N-Acetyl-5-Methoxytryptamine
Lieferbar
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Melatonin (MT). No significant cross-reactivity or interference between Melatonin (MT) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melatonin (MT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melatonin (MT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Melatonin (MT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Melatonin (MT) and unlabeled Melatonin (MT) (Standards or samples) with the pre-coated antibody specific to Melatonin (MT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Melatonin (MT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Melatonin (MT) in the sample.
Manufacturer - Research Area	Metabolic pathway; Tumor immunity; Neuro science; Hormone metabolism;

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