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ELISA Kit for Aldosterone (ALD)

ELISA Kit for Aldosterone (ALD)

ArtNr	CEA911Ge-5x96T
Hersteller	Cloud-Clone
Menge	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Food Safety Proteins Metabolism Diabetes ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Breast Cancer
Typ	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 9.41pg/mL
Citations	High Salt Intake Down-Regulates Colonic Mineralocorticoid Receptors, Epithelial Sodium Channels and 11β-Hydroxysteroid Dehydrogenase Type 2 Evidence for glucocorticoid&-mediated hypertension after uninephrectomy Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach. Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction. Magnitude of Resistant Hypertension and Impact of Aldosterone to Renin Ratio In Resistant Hypertension Effects of renal denervation on monocrotaline induced pulmonary remodeling Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy Regeneration of Functional Adrenal Tissue Following Bilateral Adrenalectomy. Acute adrenal cortex injury during cardiopulmonary bypass in a canine model ANP-stimulated sodium secretion in the collecting duct prevents sodium retention in renal adaptation to acid load MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Preventive preclinical efficacy of intravenously administered sphingosine-1-phosphate (S1P) in strengthening hypoxia adaptive responses to acute and sub-chronic … The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice Decreased KLHL3 expression is involved in the activation of WNK-OSR1/SPAK-NCC cascade in type 1 diabetic mice
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Lieferbar
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Aldosterone (ALD). No significant cross-reactivity or interference between Aldosterone (ALD) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldosterone (ALD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldosterone (ALD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to aldosterone has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled aldosterone analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of aldosterone in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of aldosterone in the sample.
Manufacturer - Research Area	Metabolic pathway; Endocrinology; Hormone metabolism;

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