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Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®

Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®

ArtNr	BOS-EK0642
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Food Safety Allergenes Neuroscience Neuroinflammation Aging & Neurological Disorders ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
Sequence	Belongs to the thrombospondin family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Thrombospondin-2,THBS2,TSP2
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	129991 MW
Gene Name	THBS2
Gene Full Name	Thrombospondin-2
Manufacturer - Specificity	Natural and recombinant human TSP2
Background	Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.
Immunogen	Expression system for standard: NS0; Immunogen sequence: G19-I1172
Protein Name	Thrombospondin-2
Protein Function	Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.
Description	Human TSP2 ELISA Kit PicoKine® (96 Tests). Quantitate Human THBS2 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	High expression in invertebral disk tissue.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
Sequence Similarities	Belongs to the thrombospondin family.
Sample Data	Concentration (pg/ml) \| 0 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 \| 20000 \| 40000 O.D. \| 0.025 \| 0.110 \| 0.191 \| 0.343 \| 0.605 \| 0.977 \| 1.630 \| 2.067
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 234 \| 1702 \| 6045 \| 222 \| 1773 \| 6515 Standard deviation \| 17.55 \| 789 \| 411.06 \| 17.76 \| 117.54 \| 501.65 CV (%) \| 7.5% \| 4.6% \| 6.8% \| 8% \| 6.5% \| 7.7%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 234 \| 245 \| 217 \| 210 \| 226 \| 13.79 \| 6.1% Sample 2 \| 1702 \| 1693 \| 1719 \| 1783 \| 1724 \| 35.18 \| 2% Sample 3 \| 6045 \| 5875 \| 6096 \| 5562 \| 5894 \| 208.67 \| 3.5% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	40 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK0642.pdf

BOS-EK0642-datasheet.pdf

BOS-EK0642-safety-datasheet.pdf