Vitellogenin ELISA

-70°C

Vitellogenin ELISA

_III_ Cyprinus carpis_IIII_

1. Antibody-coated microtiter plate (8wells x 12) 1 plate
2. The standard, Carp Vg. 1 ug / mL 300 uL x 2 viales
3. Dilution solution 30 mL
4. HRP-conjugated anti Carp Vg antibody (x.500) 30uL
5. Substrate buffer 15 mL
6. OPD (o-phenylendiamin) tablets 2 tab.
7. Wash solution (x 20) 30 mL
8. Stop solution 6 mL

[Supplementary]
Sandwich
[Applicable sample]
Plasma
[Other]
Dynamic range : 7.8 - 500 ng / mL
Principle
This kit is constructed using the antibodies specific to carp Vg , as both capture and secondary antibodies of sandwich ELISA format. During the assay, carp Vg in the samples is captured by the antibody, immobilized on the surface of microplate wells, and simultaneously reacts with the horseradish peroxidase (HRP) labeled antibody. Quantitation of carp Vg in samples can be made by measurement of HRP activity of the complex, which is exponential to the quantity of carp Vg.