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Mini Samples ELISA Kit for Prostaglandin E2 (PGE2)

Mini Samples ELISA Kit for Prostaglandin E2 (PGE2)

ArtNr	MEA538Ge-48T
Hersteller	Cloud-Clone
Menge	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	ELISA & Immunoassays ELISA Kits
Typ	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 9.29pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PG-E2
Lieferbar
Specificity	Pan-species (General)
Quantity options
Detection range	24.69-2, 000pg/mL
Organism species	Pan-species (General)
Sensitivity	The minimum detectable dose of this kit is typically less than 9.22pg/mL
Sample type	Serum, plasma and other biological fluids
Assay length	3h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Mini Samples Prostaglandin E2 (PGE2). No significant cross-reactivity or interference between Mini Samples Prostaglandin E2 (PGE2) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Prostaglandin E2 (PGE2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Prostaglandin E2 (PGE2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Prostaglandin E2 (PGE2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Prostaglandin E2 (PGE2) and unlabeled Mini Samples Prostaglandin E2 (PGE2) (Standards or samples) with the pre-coated antibody specific to Mini Samples Prostaglandin E2 (PGE2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Prostaglandin E2 (PGE2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Prostaglandin E2 (PGE2) in the sample.
Research Area	Metabolic pathway; Infection immunity; Endocrinology; Hormone metabolism;

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