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Mini Samples ELISA Kit for Glucagon Like Peptide 1 (GLP1)

Mini Samples ELISA Kit for Glucagon Like Peptide 1 (GLP1)

ArtNr	MEA804Mi-48T
Hersteller	Cloud-Clone
Menge	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	ELISA & Immunoassays ELISA Kits
Typ	Elisa-Kit
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Rabbit (Oryctolagus cuniculus), Dog (Canine, Canis lupus familiaris), Cow (Bos taurus), Cattle (Bovine)
Sensitivity	The minimum detectable dose of this kit is typically less than 4.24pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Lieferbar
Specificity	Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle)
Quantity options
Detection range	12.35-1, 000pg/mL
Organism species	Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle)
Sensitivity	The minimum detectable dose of this kit is typically less than 4.64pg/mL
Sample type	Serum, plasma and other biological fluids
Assay length	3h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Mini Samples Glucagon Like Peptide 1 (GLP1). No significant cross-reactivity or interference between Mini Samples Glucagon Like Peptide 1 (GLP1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Glucagon Like Peptide 1 (GLP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Glucagon Like Peptide 1 (GLP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Glucagon Like Peptide 1 (GLP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Glucagon Like Peptide 1 (GLP1) and unlabeled Mini Samples Glucagon Like Peptide 1 (GLP1) (Standards or samples) with the pre-coated antibody specific to Mini Samples Glucagon Like Peptide 1 (GLP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Glucagon Like Peptide 1 (GLP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Glucagon Like Peptide 1 (GLP1) in the sample.
Research Area	Endocrinology; Cardiovascular biology; Hepatology; Hormone metabolism;

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