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Mini Samples ELISA Kit for Peptide YY (PYY)

Mini Samples ELISA Kit for Peptide YY (PYY)

ArtNr	MEB067Mu-24T
Hersteller	Cloud-Clone
Menge	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	ELISA & Immunoassays ELISA Kits
Typ	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.35pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PYY1; Peptide tyrosine tyrosine
Lieferbar
Specificity	Mus musculus (Mouse)
Quantity options
Detection range	12.35-1, 000pg/mL
Organism species	Mus musculus (Mouse)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.35pg/mL
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Mini Samples Peptide YY (PYY). No significant cross-reactivity or interference between Mini Samples Peptide YY (PYY) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Peptide YY (PYY) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Peptide YY (PYY) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Peptide YY (PYY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Peptide YY (PYY) and unlabeled Mini Samples Peptide YY (PYY) (Standards or samples) with the pre-coated antibody specific to Mini Samples Peptide YY (PYY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Peptide YY (PYY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Peptide YY (PYY) in the sample.
Research Area	Neuro science; Hepatology;

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