Human Phospho-LYN (Y507) ELISA Kit

Lyn

-20°C

Tissue Lysates, Cell Lysates

Colorimetric

96-well Microplate

Pre-Coated 96-well Strip Microplate
Wash Buffer
Biotinylated Anti-Phosphotyrosine Antibody
Stop Solution
Assay Diluent(s)
Positive Control Sample
Lysis Buffer
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Prepare all reagents and samples as instructed in the manual.
Add 100 µl of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared 1
X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

41116158

The antibody pair provided in this kit recognizes Human LYN phosphorylated at site Tyrosine-507.

RayBio® Phospho-LYN (Y507) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated LYN protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-LYN. An anti-pan LYN antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and LYN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-LYN (Y507) antibody is used to detect phosphorylated LYN. After washing away unbound antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LYN (Y507) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

K562 cells were untreated or treated with PV. Cell lysates were analyzed using this phosphoELISA and Western Blot.