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ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)

ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)

ArtNr	SEA011Hu-10x96T
Hersteller	Cloud-Clone
Menge	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Neuronal Cell Markers
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.061ng/mL
Citations	Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … A new comorbidity model and the common pathological mechanisms of migraine and epilepsy Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclists Establishment and evaluation of a novel mouse model of peri/postmenopausal depression Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression Association of neuronal repair biomarkers with delirium among survivors of critical illness CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic children Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by … Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord … Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Neurotrophin,Abrineurin
Lieferbar
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity; Neuro science;

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