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ELISA Kit for Insulin Like Growth Factor 1 (IGF1)

ELISA Kit for Insulin Like Growth Factor 1 (IGF1)

ArtNr	SEA050Ra-96T
Hersteller	Cloud-Clone
Menge	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite By Cell Type Sarcoma Cell Biology Cell Culture Cytokines ELISA Endocrine Regulation of Lipid Metabolism ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Uterine, Ovarian & Cervical Cancer Neuronal Cell Markers
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.065ng/mL
Citations	A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapyCharacterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male ratsThe vagus nerve as a regulator of growth hormone secretionInsulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in RatsPhysical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress.The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass modelInsulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in RatsProtective Effect of Amlodipine on Rat Bone Tissue after OrchidectomyBMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopauseThe effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar ratsNegative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal MuscleThe effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary SyndromeEffects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesThe Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat ModelProtective effect of vitamin D against hyperoxia‐induced lung injury in newborn ratsA single bout of resistance exercise improves memory consolidation and increases the expression of synaptic proteins in the hippocampusParadoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training.Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via theMAPK signaling pathway.Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic EpilepsyResistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic EpilepsyEffects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPreventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …BST Stimulation Induces Atrophy and Changes in Aerobic Energy Metabolism in Rat Skeletal Muscles—The Biphasic Action of Endogenous GlucocorticoidsFenugreek Stimulates the Expression of Genes Involved in Milk Synthesis and Milk Flow through Modulation of Insulin/GH/IGF-1 Axis and Oxytocin SecretionTherapeutic effects of organic zinc on reproductive hormones, insulin resistance and
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IGFI,IGF1A,IBP1,MGF,Somatomedin C,Mechano Growth Gactor
Lieferbar
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Tumor immunity; Endocrinology;

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