ELISA Kit for Interleukin 6 (IL6)

7.8-500pg/mL

The minimum detectable dose of this kit is typically less than 3.2pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia; Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa; Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages ; The association of interleukin-16 polymorphisms with IL-16 serum levels and risk of colorectal and gastric cancer; Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance; The change of serum level of pigment epithelium-derived factor in coronary heart disease; Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing; Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity; Inflammatory markers and haptoglobin polymorphism in Saudi with non-insulin-dependent diabetes mellitus.; Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats.; Immunogenic alteration in laparoscopic common bile duct exploration.; Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy; A new signal amplification strategy of photoelectrochemical immunoassay for highly sensitive interleukin-6 detection based on TiO2/CdS/CdSe dual co-sensitized structure; MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells; Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease; Enhanced invasion of lung adenocarcinoma cells after co-culture with THP-1-derived macrophages via the induction of EMT by IL-6; GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes.; Serum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomy; Plasma CTRP-3 concentrations in Chinese patients with obesity and type II diabetes negatively correlate with insulin resistance; Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in China; Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+; The immunomodulating effect of seminal plasma on T cells; Human mesenchymal stem cells attenuate experimental bronchopulmonary dysplasia induced by perinatal inflammation and hyperoxia; Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study; miR-1934, downreCavia (Guinea pig )lated in obesity, protects against low-grade inflammation in adipocytes; Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate; Plasma Periostin Levels Are Increased in Chinese Subjects with Obesity and Type 2 Diabetes and Are Positively Correlated with Glucose and Lipid Parameters; Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates; Anti‐inflammatory properties of tianeptine on lipopolysaccharide‐induced changes in microglial cells involve toll‐like receptor‐related pathways; Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.; Plasma C1q/TNF-Related Protein-3 (CTRP-3) and High-Mobility Group Box-1 (HMGB-1) Concentrations in Subjects with Prediabetes and Type 2 Diabetes; Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats; Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection.; Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy; Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines; ВПЛИВ КРОВОВТРАТИ НА ДИНАМІКУ ВМІСТУ ЦИТОКІНІВ СИРОВАТКИ КРОВІ В ПЕРІОД ГОСТРОЇ РЕАКЦІЇ НА КРАНІОСКЕЛЕТНУ ТРАВМУ; Interleukin-6 downregulated vascular smooth muscle cell contractile proteins via ATG4B-mediated autophagy in thoracic aortic dissection; Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models; Glucosamine oral administration as an adjunct to hyaluronic acid injection in treating temporomandibular joint osteoarthritis; The effect of restorative materials on cytokines in gingival crevicular fluid; Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients; Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients; Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer; Inhibition of Fusarium solani Infection in Murine Keratocytes by Lactobacillus salivarius ssp. salivarius JCM1231 Culture Filtrate In Vitro; Chronic Osteomyelitis Increases the Incidence of Type 2 Diabetes in Humans and Mice; Tanshinone II is a potent candidate for treatment of lipopolysaccharide-induced acute lung injury in rat model; Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children; Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats ; Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women; Blocking the Feedback Loop between Neuroendocrine Differentiation and Macrophages Improves the Therapeutic Effects of Enzalutamide (MDV3100) on Prostate Cancer.; Attenuation of acute systemic inflammatory response after valve surgery; Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort.; Reciprocal network between cancer stem-like cells and macrophages facilitates the progression and androgen deprivation therapy resistance of prostate cancer; Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus; Polar extract of protects cartilage homeostasis: possible mechanism of action; PPARγ alleviated hepatocyte steatosis through reducing SOCS3 by inhibiting JAK2/STAT3 pathway; Uncoupling Protein 2 and Peroxisome Proliferator-Activated Receptor γ Gene Polymorphisms in Association with Diabetes Susceptibility in Chinese Han Population …; Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4) …; Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage; Comparison of Plasma Interleukin-6 and Interleukin-10 Concentrations between Female Dogs with Mammary Gland Tumours and Healthy; Effects of different doses of ticagrelor on platelet aggregation and endothelial function in diabetic patients with stable coronary artery disease; Honokiol protects pulmonary microvascular endothelial barrier against lipopolysaccharide-induced ARDS partially via the Sirt3/AMPK signaling axis; Activated hepatic stellate cells promote epithelial-to-mesenchymal transition in hepatocellular carcinoma through transglutaminase 2-induced pseudohypoxia; Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells; Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD; Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard &; Long non-coding RNA mitigates atherosclerosis by regulating the actin-binding protein NEXN; Astragaloside IV regulates the PI3K/Akt/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation; Improving the anti-keloid outcomes through liposomes loading paclitaxel–cholesterol complexes; Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling; The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency; Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus; miR-21 promotes proliferation and inhibits apoptosis of hepatic stellate cells through targeting PTEN/PI3K/AKT pathway; Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammation; Inflammatory anemia-associated parameters are related to 28-day mortality in patients with sepsis admitted to the ICU: a preliminary observational study; Lanthanum chloride induces neuron damage by activating the nuclear factor-kappa B signaling pathway in activated microglia; STAT3 polymorphisms and IL-6 polymorphism are associated with the risk of basal cell carcinoma in patients from northern Poland;