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ELISA Kit for Leptin (LEP)

ELISA Kit for Leptin (LEP)

ArtNr	SEA084Po-96T
Hersteller	Cloud-Clone
Menge	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Obesity ELISA Endocrine Regulation of Lipid Metabolism ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Pig (Porcine, Sus scrofa domesticus), Wild Boar
Sensitivity	The minimum detectable dose of this kit is typically less than 0.113ng/mL
Citations	Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrientsand Growth, but Also Body Composition and Endocrinological Parameters –Study on Piglets Used As a Model for Children Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. Evaluating measures of exploratory behaviour in sows around farrowing and during lactation− A pilot study Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	OB,OBS,Obesity Homolog,Obesity Factor,Obese Protein
Lieferbar
Specificity	Sus scrofa; Porcine (Pig)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Sus scrofa; Porcine (Pig)
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Leptin (LEP). No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leptin (LEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leptin (LEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leptin (LEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leptin (LEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leptin (LEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Metabolic pathway; Endocrinology; Cardiovascular biology;

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