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ELISA Kit for Nerve Growth Factor (NGF)

ELISA Kit for Nerve Growth Factor (NGF)

ArtNr	SEA105Ra-10x96T
Hersteller	Cloud-Clone
Menge	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Neuroscience Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.9pg/mL
Citations	Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism Antidepressant drugs for beta amyloid-induced depression: A new standpoint? N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … Neurobiological determinants of depressive-like symptoms in rodents Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	NGFB,Beta-NGF,HSAN5,NGF-B,Beta-Nerve Growth Factor
Lieferbar
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF). No significant cross-reactivity or interference between Nerve Growth Factor (NGF) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor (NGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nerve Growth Factor (NGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nerve Growth Factor (NGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nerve Growth Factor (NGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Neuro science;

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