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ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)

ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)

ArtNr	SEA133Bo-96T
Hersteller	Cloud-Clone
Menge	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Uterine, Ovarian & Cervical Cancer
Typ	Elisa-Kit
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	The minimum detectable dose of this kit is typically less than 3.1pg/mL
Citations	Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steersThe Concentrations of Inflammatory Cytokines and Acute‐Phase Proteins in the Peripheral Blood and Uterine Washings in Cows with PyometraInflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum.Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranesInflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartumInflammatory cytokine and acute phase protein concentrations in the peripheral blood and uterine washings of cows with subclinical endometritis in the late postpartum periodPathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos …MAVS induces a host cell defense to inhibit CSFV infectionMetabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cowsLevels of selected cytokines and acute–phase proteins in the serum of dairy cows with cystic ovarian disease and those in follicular and luteal phases of normal …Variations in milk production based on the temperature-humidity index and blood metabolic parameters in cows during exposure to heat stress.Early cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattleEffective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale ExtractsAdipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactationEvaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cowsHepatic nuclear factor kappa B signaling pathway and NLR family pyrin domain containing 3 inflammasome is over-activated in ketotic dairy cowsEffect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein …The association of circulating prepartum metabolites, minerals, cytokines and hormones with postpartum health status in dairy cattleMitochondrial membrane protein mitofusin 2 as a potential therapeutic target for treating free fatty acid–induced hepatic inflammation in dairy cows during early …Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cowsFasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Association Between Serum Selenium Level and Subclinical Mastitis in Dairy CattlePeripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …Integrated effects of season and parturition-associated stress on the inflammatory response and metabolic status in Sahiwal (Bos Indicus) cowsГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИHormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation PeriodTHE CHANGE OF INDICATORS OF PROTEIN, LIPID AND CARBOHYDRATE METABOLISM IN DAIRY COWS AFTER CALVING DURING THE FORMATION OF …Metabolic adaptation in first week after calving and early prediction of ketosis type I and II in dairy cowsEffect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calvesInfluence of niacin application on inflammatory parameters, non-esterified fatty acids and functional status of liver in cows during early lactationComparative evaluation of cytokine and acute-phase protein concentrations in sera of dairy cows with subclinical and clinical ketosis as a different view of the ¡Optimizing the growth and immune system of dairy calves by subdividing the pre-weaning period and providing different milk volumes for each stageThe Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant ModelOveractivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB …Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	DIF,TNF-A,TNFSF2,Cachectin,Tumor Necrosis Factor Ligand Superfamily Member 2
Lieferbar
Specificity	Bos taurus; Bovine (Cattle)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Tumor immunity; Infection immunity;

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