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ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10)

ArtNr SEA371Hu-10x96T
Hersteller Cloud-Clone
Menge 10x96 T
Quantity options 10x96 T 24 T 48 T 5x96 T 96 T
Kategorie
Typ Elisa-Kit
Specific against Human (Homo sapiens)
Sensitivity The minimum detectable dose of this kit is typically less than 5.5pg/mL
Citations Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors
IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancer
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10
Lieferbar
Specificity Homo sapiens (Human)
Quantity options
Detection range
15.6-1, 000pg/mL
Organism species
Homo sapiens (Human)
Sensitivity
The minimum detectable dose of this kit is typically less than 5.6pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity

This assay has high sensitivity and excellent specificity for detection of Interferon Gamma Induced Protein 10kDa (IP10).
No significant cross-reactivity or interference between Interferon Gamma Induced Protein 10kDa (IP10) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma Induced Protein 10kDa (IP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma Induced Protein 10kDa (IP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma Induced Protein 10kDa (IP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Gamma Induced Protein 10kDa (IP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Cytokine; Tumor immunity; Infection immunity;
Reference

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10x96 T
Lieferbar: In stock
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