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ELISA Kit for High Mobility Group Protein 1 (HMGB1)

ELISA Kit for High Mobility Group Protein 1 (HMGB1)

ArtNr	SEA399Hu-48T
Hersteller	Cloud-Clone
Menge	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Neural Transcription Factors Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Neural Signaling Protein Aggregation, Clearance & Misfolding By Cell Type Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer Bladder Cancer Brain Cancer Stem Cell Markers
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 28.3pg/mL
Citations	High mobility group box protein-1 (HMGB-1) as a new diagnostic marker in patients with acute appendicitisClinical relevance of single nucleotide polymorphisms of the high mobility group box 1 protein gene in patients with major trauma in Southwest ChinaIs HMGB1 a New Indirect Marker for Revealing Fibrosis in Chronic Hepatitis and a New Therapeutic Target in Treatment?Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosisHigh mobility group B1 levels in sepsis and disseminated intravascular coagulationElevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity and quality of lifeIGF-1 alleviates ox-LDL-induced inflammation via reducing HMGB1 release in HAECs Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 ExpressionCorrelation between serum high-mobility group box-1 levels and high-sensitivity C-reactive protein and troponin I in patients with coronary artery diseaseSerum levels of high mobility group box 1 protein and its association with quality of life and psychological and functional status in patients with fibromyalgiaSoluble receptor for advanced glycation end-products and progression of airway diseaseEffects of Single or Combined Treatments with Radiation and Chemotherapy on Survival and Danger Signals Expression in Glioblastoma Cell LinesHigh plasma levels of high mobility group box 1 is associated with the risk of sepsis in severe blunt chest trauma patients: a prospective cohort studyThe Alarmin Concept Applied to Human Renal Transplantation: Evidence for a Differential Implication of HMGB1 and IL-33Plasma levels of high mobility group box 1 increase in patients with posttraumatic stress disorder after severe blunt chest trauma: a prospective cohort studyHigh-mobility group box-1, promising serological biomarker for the distinction of human WNV disease severityIncreased VEGF and Decreased SDF-1α in Patients with Silent Brain Infarction Are Associated with Better Prognosis after First-Ever Acute Lacunar Stroke Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityPlasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in ChinaHigh mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgeryOverexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitroLow Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-1 Pathway to Induce Inflammation ResponseSerum levels of HMGB1 have a diagnostic role in metastatic renal cell cancerHigh mobility group box1 protein is involved in acute inflammation induced by Clostridium difficile toxin A.COMPOSITIONS AND METHODS FOR INHIBITING HMGB1 ACTIVATION OF MELANOCYTESMETHOD OF MAKING A SKIN CARE COMPOSITIONRelevance of pre-analytical blood management on the emerging cardiovascular protein biomarkers TWEAK and HMGB1 and on miRNA serum and plasma profiling.Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases.Changes in pro-inflammatory markers and leucine concentrations in response to Nordic Walking training combined with vitamin D supplementation in elderly women.Serum HMGB1 serves as a novel laboratory indicator reflecting disease activity andHMGB1 blockade differentially impacts pulmonary inflammation and defense responses in poly (I: C)/LPS-exposed heart transplant miceThe role of high mobility group box 1 protein in interleukin-18-induced myofibroblastic transition of valvular interstitial cellsUrinary MCP-1、HMGB1 increased in calcium nephrolithiasis patients and the influence ofhypercalciuria on the production of the two cytokines.Diagnostic Value of High-Mobility Group Box 1 (HMGB1) Protein in Acute and Perforated Appendicitis.High Mobility Group Box 1 Mediates Interferon-r-Induced Phenotypic Modulation of Vascular Smooth Muscle Cells.Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells.SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death.The expression of HMGB1 and the receptor for advanced glycation end products (RAGE) in epithelial ovarian cancerSIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from deathInduction of Immunogenic Cell Death in Lymphoma Cells by Wharton’s Jelly Mesenchymal Stem Cell Conditioned MediumOxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 releaseCorrelation between high mobility group box 1 protein, tumor necrosis factor-α, and acute coronary syndromePERK induces resistance to cell death elicited by endoplasmic reticulum stress and chemotherapyAssessing the recovery from prerenal and renal acute kidney injury after treatment with single herbal medicine via activity of the biomarkers HMGB1, NGAL and KIM-1 in kidney proximal tubular cells treated by cisplatin with different doses and exposure times.Blocking the Feedback Loop between Neuroendocrine Differentiation and Macrophages Improves the Therapeutic Effects of Enzalutamide (MDV3100) on Prostate Cancer.Secreted tryptophanyl-tRNA synthetase as a primary defence system against infection.Bromodomain inhibition exerts its therapeutic potential in malignant pleural mesothelioma by promoting immunogenic cell death and changing the tumor immune …Tumor lysate-based vaccines: on the road to immunotherapy for gallbladder cancerOridonin enhances the cytotoxicity of 5-FU in renal carcinoma cells by inducting necroptotic deathThrombomodulin, alarmin signaling, and copeptin: cross-talk between obesity and acute ischemic stroke initiation and severity in EgyptiansIncreased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsiaMolecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary DiseaseHMGB1-RAGE signaling pathway in pPROMChronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypesiris-AperTODulaglutide mitigates inflammatory response in fibroblast-like synoviocytesBET bromodomain inhibitor JQ1 promotes immunogenic cell death in tongue squamous cell carcinomaHigh mobility group box protein 1 neutralization therapy in ovine bacteremia: Lessons learned from an ovine septic shock model incorporating intensive care supportEvaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters …HMGB1 Mediates Paraquat-Induced Neuroinflammatory Responses via Activating RAGE Signaling PathwayDamage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortDendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In VitroA novel diagnostic tool for the detection of bladder cancer: Measurement of urinary high mobility group box-1Marathon Run-induced Changes in the Erythropoietin-Erythroferrone-Hepcidin Axis are Iron DependentGlycyrrhizin Prevents Hemorrhagic Transformation and Improves Neurological Outcome in Ischemic Stroke with Delayed Thrombolysis Through Targeting …Hypoxic Wharton's Jelly Stem Cell Conditioned Medium Induces Immunogenic Cell Death in Lymphoma CellsAberrant Expression of High Mobility Group Box Protein 1 in the Idiopathic Inflammatory MyopathiesNaja sumatrana venom cytotoxin, sumaCTX exhibits concentration-dependent cytotoxicity via caspase-activated mitochondrial-mediated apoptosis without …DAMPs released by pyroptotic cells as major contributors and therapeutic targets for CAR-T-related toxicitiesInsights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell DeathAutophagy-associated HMGB-1 as a novel potential circulating non-invasive diagnostic marker for detection of Urothelial Carcinoma of BladderClinical significance of high mobility group box 1/toll-like receptor 4 in obese diabetic patientsIntracellular translocation of HMGB1 is important for Zika virus replication in Huh7 cellsHigh Mobility Group Box-1 Protein and Interleukin 33 Expression in Allergic RhinitisSKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesotheliomaAbnormal intestinal milieu in post-traumatic stress disorder is not impacted by treatment that improves symptomsThe antiviral action of resveratrol against Zika virus through the regulation of interferon responses by HMGB1
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	HMG1,HMG3,SBP1,Sulfoglucuronyl Carbohydrate Binding Protein,Amphoterin,High Mobility Group Box 1 Protein
Lieferbar
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMGB1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMGB1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMGB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMGB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMGB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMGB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Protein 1 (HMGB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group Protein 1 (HMGB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Infection immunity;

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