« Zurück
Startseite /
ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)

ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)

ArtNr	SEA548Ra-24T
Hersteller	Cloud-Clone
Menge	24 Tests
Quantity options	10x96 Tests 24 Tests 48 Tests 5x96 Tests 96 Tests
Kategorie	ELISA & Immunoassays ELISA Kits
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.127ng/mL
Citations	Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in RatsEFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATSAstragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression.Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPeroxisome Proliferator-activated Receptor-γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive RatsEffect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley ratsEffect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidismAllopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in ratsSubchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationA Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in RatsProtective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusionChanges in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil.Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo studyGlobal gene expression profile of cerebral ischemia-reperfusion injury in rat MCAO modelAspirin modulates the inflammatory response in a thrombus‑stimulated LMVEC modelDiallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathwayDetoxification and activating blood circulation decoction reduces restenosis involving the TLR4/NF-κB pathway after balloon injuryCellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial DysfunctionVanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemiaGinger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in ratsAnti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat ModelDehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD54,BB2,P3.58,Human Rhinovirus Receptor,Major group rhinovirus receptor
Lieferbar
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 1 (ICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intercellular Adhesion Molecule 1 (ICAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	CD & Adhesion molecule; Tumor immunity; Infection immunity

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.