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ELISA Kit for Caspase 3 (CASP3)

ELISA Kit for Caspase 3 (CASP3)

ArtNr	SEA626Ra-24T
Hersteller	Cloud-Clone
Menge	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	ELISA & Immunoassays ELISA Kits
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.060ng/mL
Citations	Protective Effects of Morus alba Leaves Extract on Ocular Functions of Pups from Diabetic and Hypercholesterolemic Mother RatsThe combined effect of metformin and L-cysteine on inflammation, oxidative stress and insulin resistance in streptozotocin-induced type 2 diabetes in ratsAttenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelAmelioration of nandrolone decanoate-induced testicular and sperm toxicity in rats by taurine: Effects on steroidogenesis, redox and inflammatory cascades, and intrinsic apoptotic pathwayAntioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in ratsVitamin D ameliorates hepatic ischemic/reperfusion injuryin ratsApoptotic effects of dipyrido [3,2-a:2',3'-c] phenazine (dppz) Au(III) complex against diethylnitrosamine/phenobarbital induced experimental hepatocarcinogenesis in ratsPomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in ratsNeuroprotective effects of rosuvastatin against traumatic spinal cord injury in ratsSaxagliptin: A novel antiparkinsonian approachUrsodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial PerturbationsProtective role of ellagitannins from Eucalyptus citriodora against ethanol-induced gastric ulcer in rats: Impact on oxidative stress, inflammation and calcitonin-gene related peptideEpigallocatechin-3-gallate pretreatment attenuates doxorubicin-induced cardiotoxicity in rats: A mechanistic studyCILOSTAZOL HEPATOPROTECTIVE EFFECT AGAINST ISCHEMIA/REPERFUSION:INVOLVEMENT OF GSK-3β, CYCLIN D1 and WNT/β-CATENIN PATHWAYNeuroprotectiveAntidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting DrugBDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 CellsGeraniol ameliorates TNBS-induced colitis: Involvement of Wnt/棺-catenin, p38MAPK, NF觀B, and PPAR款 signaling pathwaysCrocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathwaysCorrelation of In Vivo and In Vitro Assay Results for Assessment of Free Radical Scavenging Activity of Green Tea NutraceuticalsNeuroprotective effects of atomoxetine against traumatic spinal cord injury in ratsTHE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATSArecoline Induces Neurotoxicity to PC12 Cells: Involvement in ER Stress and Disturbance of Endogenous H2S GenerationNeuroprotective Effects of Pregabalin Against Spinal Cord Ischemia-Reperfusion Injury in RatsThe hepatocurative effects of Cynara scolymus L. leaf extract on carbon tetrachloride-induced oxidative stress and hepatic injury in ratsAntioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats.Dapoxetine attenuates testosterone-induced prostatic hyperplasia in rats by the regulation of inflammatory and apoptotic proteinsArecoline Induces Neurotoxicity to PC12 Cells: Involvement in ER Stress and Disturbance of Endogenous H2S Generation.Chrysin attenuates testosterone-induced benign prostate hyperplasia in ratsRole of Pomegranate Juice and Atorvastatin in Ameliorating Spinal Neurotoxicity of Wistar Rats Maternally Fed on Hypercholesterolemic DietA Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in RatsA polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in ratsModulatory effect of silymarin on nuclear factor-erythroid-2-related factor 2 regulated redox status, nuclear factor-κB mediated inflammation and apoptosis in experimental gastric ulcerDocking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus.Nephroprotective effect of saxagliptin against gentamicin-induced nephrotoxicity, emphasis on anti-oxidant, anti-inflammatory and anti-apoptic effectsPosttreatment Effects of L. Leaf Extract on Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in RatsAnti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspringEnoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosisEffect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in RatsPotential immunomodulatory and antioxidant effects of walnut vegetable oil against lead‐mediated hepatic damage and their interaction with lipase …Adiponectin attenuates lipopolysaccharide-induced cell injury of H9c2 cells by regulating AMPK pathwayGum Acacia mitigates diclofenac nephrotoxicity by targeting monocyte chemoattractant protein-1, complement receptor-1 and pro-apoptotic pathwaysTherapeutic effects of nano-HAp in a rat model of AlCl3 induced neurotoxicityProphylactic Impact of Beta vulgaris L in Ameliorating Cyclosporine A-Induced Hepatotoxicity in Rats.Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.Dietary supplementation of barley and/or dates attenuate hypercholesterolemic‐induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic …The Role of Oxidative Stress, Renal Inflammation, and Apoptosis in Post Ischemic Reperfusion Injury of Kidney Tissue: the Protective Effect of Dose-Dependent Boric …Betaine suppresses cell proliferation by increasing oxidative stress–mediated apoptosis and inflammation in DU-145 human prostate cancer cell lineCarnosine and l-arginine attenuate the downregulation of brain monoamines and gamma aminobutyric acid reverse apoptosis and upregulate the expression of …Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in ratsCurcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway.Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic …Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in ratStrawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male ratsAccacia senegel extract ameliorates Lead Acetate-mediated Hepatotoxicity in rats.Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar ratsConcanavalin A induces apoptosis in a dose©\dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cellsCyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cellsCaptopril downregulates expression of Bax/cytochrome C/caspase-3 apoptotic pathway, reduces inflammation, and oxidative stress in cisplatin-induced acute?¡Reproductive Effects of Nicotinamide on Testicular Function and Structure in Old Male Rats: Oxidative, Apoptotic, Hormonal, and Morphological AnalysesEffect of the standard herbal preparation, STW5, treatment on dysbiosis induced by dextran sodium sulfate in experimental colitisComparative effects of metformin and Cistus laurifolius L. extract in streptozotocin-induced diabetic rat model: oxidative, inflammatory, apoptotic, and histopathological?¡Piceatannol Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Modulation of Nrf2/HO-1/NF¦ÊB Axis. Front. Pharmacol. 11: 614897. doi?¡Protective role of naringin loaded solid nanoparticles against aflatoxin B1 induced hepatocellular carcinomaThe Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in MicrogliaChrysin attenuates estradiol-induced endometrial hyperplasia in rats via enhancing PPARα activityNeuroprotective effect of sodium alginate against chromium-induced brain damage in ratsEthnopharmacological impact of Melaleuca rugulosa (Link) Craven leaves extract on liver inflammationOksidatif, Apoptotik ve İnflamatuar Sinyal Yolakları üzerinden C6 Glioblastoma Hücrelerindeki ML351'in Antiproliferatif EtkileriPromising Therapeutic Efficacy of Trigonella-foenum graecum and Bone Marrow-Derived Mesenchymal Stem Cells on Skeletal Muscle Atrophy in Experimental …Potential activity of silver/hydroxyapatite nano composite against damaged liver consequence to irradiation
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CPP32,CPP32B,SCA1,Apoptain,Yama,Apoptosis-Related Cysteine Peptidase,Cysteinyl Aspartate Specific Proteinases 3,SREBP cleavage activity 1
Lieferbar
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3). No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 3 (CASP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 3 (CASP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 3 (CASP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 3 (CASP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Signal transduction; Enzyme & Kinase; Apoptosis; Tumor immunity; Infection immunity

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