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ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)

ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)

ArtNr	SEA837Mu-96T
Hersteller	Cloud-Clone
Menge	96 Tests
Quantity options	10x96 Tests 24 Tests 48 Tests 5x96 Tests 96 Tests
Kategorie	Oncology Neuroscience Metabolism Cholesterol & Lipid Metabolism Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer Colorectal Cancer Breast Cancer Head & Neck Cancer Testicular & Prostate Cancer Gastric Cancer Lymphatic System Cancer
Typ	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.116ng/mL
Citations	High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic miceHyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expressionAdenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1betaHyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptorsRegulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling PathwaysThe inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis.Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunitsInhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK/NF-kB signaling pathway and apoptosis, in SKH-1 miceProtective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunitsUtility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studiesBeta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytesProtective Effect ofAcacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in miceToxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaInfluences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiationAnti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice.Protective Effect of Zingiber officinale Against Dalton's Lymphoma Ascites Tumour byRegulating Inflammatory Mediator and Cytokines.Anti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK/NF-κB pathwayMelatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathyTargeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and …Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injuryErinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	NOS2A,INOS,HEP-NOS,I-NOS,Hepatocytes Oxide Synthase,Peptidyl-cysteine S-nitrosylase NOS2
Lieferbar
Specificity	Mus musculus (Mouse)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2). No significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 2, Inducible (NOS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 2, Inducible (NOS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Enzyme & Kinase; Metabolic pathway; Tumor immunity; Cardiovascular biology;

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