« Zurück
Startseite /
ELISA Kit for Surfactant Protein B (SP-B)

ELISA Kit for Surfactant Protein B (SP-B)

ArtNr	SEB622Hu-48T
Hersteller	Cloud-Clone
Menge	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Oncology Metabolism Diabetes ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.59ng/mL
Citations	Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgeryDetection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polypsSurfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot studyStaphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant ProteinsThe Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNSAcute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps projectSurfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart FailureNachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene AugePlasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective studySerum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE)The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions.Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid.Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal FluidDiving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional diversSurfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid ProductionSurfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SFTPB,PSPB,SFTB3,SFTP3,SPB,Surfactant Associated Protein B,Pulmonary Surfactant Protein B,18 kDa pulmonary-surfactant protein,6 kDa protein
Lieferbar
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Surfactant Protein B (SP-B). No significant cross-reactivity or interference between Surfactant Protein B (SP-B) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Protein B (SP-B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Protein B (SP-B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Protein B (SP-B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Protein B (SP-B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Protein B (SP-B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Protein B (SP-B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Tumor immunity; Infection immunity; Pulmonology;

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.