ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1)

0.156-10ng/mL

The minimum detectable dose of this kit is typically less than 0.065ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Endothelial Cell Specific Molecule 1 (ESM1).
No significant cross-reactivity or interference between Endothelial Cell Specific Molecule 1 (ESM1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelial Cell Specific Molecule 1 (ESM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelial Cell Specific Molecule 1 (ESM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation; Endocan is useful biomarker of survival and severity in sepsis.; Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes; Endocan is useful biomarker of survival and severity in sepsis; Endocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus Erythematosus; Assessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control study; Diagnostic and prognostic values of endothelial-cell-specific molecule-1 with malignant pleural effusions in patients with non-small cell lung cancer; Changes in Endocan Levels and Blood Coagulation in HIV Infection; Is the plasma endocan level a reliable predictor for the severity of erectile dysfunction?; Serum Level of Endothelial Cell-Specific Molecule-1 (ESM-1) as a New Potential Biomarker for Rheumatoid Arthritis Disease Activity; Endothelial biomarkers in the light of new sepsis definition; Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …;