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ELISA Kit for Renalase (RNLS)

ELISA Kit for Renalase (RNLS)

ArtNr	SEC845Hu-5x96T
Hersteller	Cloud-Clone
Menge	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Kategorie	Neuroscience Neural Signaling Cell Biology Cell Status ELISA Oxidative Stress ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 1.31ng/mL
Citations	Renalase, kidney function, and markers of endothelial dysfunction in renal transplant recipients Renalase, Stroke, and Hypertension in Hemodialyzed Patients Renalase in peritoneal dialysis patients is not related to blood pressure, but to dialysis vintage. Vascular adhesion protein-1 and renalase in regard to diabetes in hemodialysis patients Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients VAP-1 in chronic kidney disease and diabetic kidney disease The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy Renalase, a novel enzyme involved in blood pressure regulation, is related to kidney function but not to blood pressure in hemodialysis patients. Renalase in hypertension and kidney disease Markers of increased cardiovascular risk in patients with chronic kidney disease Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients Normal values for urine renalase excretion in children Low plasma renalase concentration in hypertensive patients after surgical repair of coarctation of aorta Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults Circulating renalase, catecholamines, and vascular adhesion Renalase levels in children with solitary functioning kidney SP321RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS PP.27.04: ENDOTHELIAL DYSFUNCTION AND ARTERIAL HYPERTENSION IN THE WORKING POPULATION (MERIDIAN STUDY). Renalase is Removed by Kidneys and During Dialysis – Excess Related to CKD Complications? Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis Plasma renalase concentration before and after radiofrequency renal denervation in patients with resistant hypertension Urinary renalase concentration in patients with preserved kidney function undergoing coronary angiography. PLASMA RENALASE CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION: PRELIMINARY RESULTS Increased serum renalase in hemodialysis patients: is it related to left ventricular hypertrophy? High-performance Liquid Chromatography Measured Metabolites of EndogenousCatecholamines and Their Relations to Chronic Kidney Disease and High Blood Pressure in Heart Transplant Recipients. Online Haemodiafiltration Improves Inflammatory State in Dialysis Patients: A Longitudinal Study. Renalase in Children with Glomerular Kidney Diseases Plasma Renalase is Not Associated with Blood Pressure and Brachial-Ankle Pulse Wave Velocity in Chinese Adults With Normal Renal Function Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls Increased serum renalase in peritoneal dialysis patients: Is it related to cardiovascular disease risk Aumento de renalasa sérica en pacientes de diálisis peritoneal:¿ está relacionado con el riesgo de enfermedad cardiovascular? Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients Levels of renalase and advanced oxidation protein products with regard to catecholamines in haemodialysed patients Endocan Concentration in Patients With Primary Hypertension RENALASE PLASMA CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION Transient changes in serum renalase concentration during long-distance running: The case of an amateur runner under continuous training Synergistic effect of renalase and chronic kidney disease on endothelin-1 in patients with coronary artery disease‒a cross-sectional study Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration A new method for quantitative determination of renalase based on mass‐spectrometry determination of a proteotypic peptide labeled with stable isotopes Serum Renalase Levels in Adolescents with Primary Hypertension Atorvastatin facilitates protection against contrast-induced nephropathy in patients undergoing coronary angiography via humoral mediators rather than … High urinary kidney injury molecule type 1 level is associated with 12-month adverse cardiovascular events in patients with coronary artery disease Serum Renalase Levels Are Predicted by Brain-Derived Neurotrophic Factor and Associated with Cardiovascular Events and Mortality after Percutaneous Coronary … GP23 Percutaneous closure of patent ductus arteriosus: single center experience GP25 Comparison of associations and outcomes of prenatally and postnatally diagnosed right aortic arch: south wales' experience GP24 Serum renalase levels in adolescents with primary hypertension Association of Plasma Renalase and Left Ventricle Mass Index in Heart Failure Patients Stratified to the Category of the Ejection Fraction: A Pilot Study The role of urinary renalase on early-stage renal damage in Chinese adults with primary hypertension The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patients Association of Serum Renalase Levels and Renalase rs10887800 Polymorphism with Unstable Angina Pectoris Patients Having Metabolic Syndrome Nikotinamidadenindinukleotid-Blutplasmawerte sind unabhängig vom kalendarischen und vaskulären Alter in einer Normalkohorte aus der Region Halle-Saalekreis Circulating Renalase as Predictor of Renal and Cardiovascular Outcomes in Pre-Dialysis CKD Patients: A 5-Year Prospective Cohort Study Edited by: Background: Renalase has been implicated in chronic heart failure (CHF) however, nothing is known about renalase discriminatory ability and prognostic …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MAO-C,C10orf59,FAD-Dependent Amine Oxidase,Monoamine oxidase-C,alpha-NAD(P)H oxidase/anomerase
Lieferbar
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Renalase (RNLS). No significant cross-reactivity or interference between Renalase (RNLS) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renalase (RNLS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renalase (RNLS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renalase (RNLS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renalase (RNLS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renalase (RNLS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Renalase (RNLS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Enzyme & Kinase;

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