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ELISA Kit for Periostin (POSTN)

ELISA Kit for Periostin (POSTN)

ArtNr	SEH339Hu-10x96T
Hersteller	Cloud-Clone
Menge	10x96 Tests
Quantity options	10x96 Tests 24 Tests 48 Tests 5x96 Tests 96 Tests
Kategorie	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer Pancreatic Cancer Lymphatic System Cancer
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 33pg/mL
Citations	Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients A Novel Marker Of Bone Strength? Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY study Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY Study Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formation Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal disease 血清内脂素水平与多囊卵巢综合征的相关性研究 Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY study Serum periostin is a potential biomarkerfor non-alcoholic fatty liver disease: a case–control study High levels of periostin correlate with increased fracture rate, diffuse MRI pattern, abnormal bone remodeling and advanced disease stage in patients with newly … Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. Circulating periostin levels increase in association with bone density loss and healing progression during the early phase of hip fracture in Chinese older women The Utility of Biomarkers in Osteoporosis Management Cortical and trabecular bone microstructure did not recover at weight-bearing skeletal sites and progressively deteriorated at non-weight-bearing sites during the year following International Space Station missions Periostin and sclerostin levels in juvenile Paget's disease Development of a New Immunoassay for Human Cathepsin K-Generated Periostin Fragments as a Serum Biomarker for Cortical Bone Characterization of a sandwich ELISA for the quantification of all human periostin isoforms Interaction between LRP5 and periostin gene polymorphisms on serum periostin levels and cortical bone microstructure Effect of age and gender on serum periostin: Relationship to cortical measures, bone turnover and hormones Association between serum periostin concentrations and outcome after acute spontaneous intracerebral hemorrhage Serum periostin concentrations and outcomes after severe traumatic brain injury Predictive and prognostic value of serum periostin in advanced non–small cell lung cancer patients receiving chemotherapy Serum Levels of a Cathepsin-K Generated Periostin Fragment Predict Incident Low-Trauma Fractures in Postmenopausal Women Independently of BMD and FRAX ОСОБЕННОСТИ СОСТОЯНИЯ ЗДОРОВЬЯ НОВОРОЖДЕННЫХ, РОЖДЕННЫХ ОТ МАТЕРЕЙ С БРОНХИАЛЬНОЙ АСТМОЙ Circulating periostin in patients with nonalcoholic fatty liver disease. The C-Terminal Intact Forms of Periostin (iPTN) Are Surrogate Markers for Osteolytic Lesions in Experimental Breast Cancer Bone Metastasis Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitus Rheumatoid arthritis in remission Myostatin and other musculoskeletal markers in lung transplant recipients Polycystic Ovary Syndrome is Associated with Elevated Periostin Levels ИСПОЛЬЗОВАНИЕ СЫВОРОТОЧНОГО ПЕРИОСТИНА В КАЧЕСТВЕ МАРКЕРА ОБОСТРЕНИЙ АСТМЫ У ДЕТЕЙ Tartrate-resistant acid phosphatase 5b, but not periostin, is useful for assessing Paget's disease of bone Identification and diagnostic value of pleural fluid periostin and serum periostin of malignant pleural effusions in patients with non–small‐cell lung cancer Serum periostin is associated with cancer mortality but not cancer risk in older home-dwelling men: A 8-year prospective analysis of the STRAMBO study Serum and placental periostin levels in women with early pregnancy loss Evaluation of periostin levels in gingival crevicular fluid and peri-implant sulcus fluid in patients with periodontal and peri-implanter disease: A cross-sectional … Myostatin and Markers of Bone Metabolism in Dermatomyositis High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease The Relationship between Serum Periostin Levels and Neutrophil¨CLymphocyte Ratio with Lesion Localization and Acute Prognosis in Acute Ischemic Stroke First Admission Neutrophil–Lymphocyte Ratio May Indicate Acute Prognosis of Ischemic Stroke
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	OSF-2,PDLPOSTN,PN,Osteoblast Specific Factor 2
Lieferbar
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Periostin (POSTN). No significant cross-reactivity or interference between Periostin (POSTN) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Periostin (POSTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Periostin (POSTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Periostin (POSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Periostin (POSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Periostin (POSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Periostin (POSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Metabolic pathway;

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