ARHGEF7 Antibody

This antibody is also available with the following conjugates:
AF350, AF405L, AF405M, AF405S, AF488, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF635, AF647, AF680, AF700, AF750, AF790, APC, AP, Biotin, Cy3, Cy5.5, Cy5, Cy7, FITC, HRP, PE, Magnetic beads (1 um, 2.8 um, 3 um, 4.5 um, 5 um, 10 um, 15 um, 20 um, 30 um, or different size option)
Please contact sales@hoelzel.de for pricing and availability.

90kDa

Affinity purification

Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/alphapix and cool1/betaPix, the latter having two splice variants that vary in their carboxy termini (1). Cool1/betaPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization. Cool1/betaPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3, 4). Phosphorylation of cool1/betaPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/betaPix, regulating formation of growth cones in response to growth factors (4). Growth factor induced activation of Rac1 via cool1/betaPix was later shown to occur independently of subcellular localization (5). Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/betaPix and activation of cdc42 (6).