Immunoprecipitation Buffer w/ Triton X-100 (TIB-1)

The Triton Immunoprecipitation and Cell Lysis Buffer is applicable to most standard immunoprecipitation procedures. It is used to lyse cells, and solubilize proteins that can be co-immunoprecipitated. The lysis buffer abbrogates hydrophobic interactions but maintains charge-charge interactions between proteins.

Synopsis of cell lysis and immunoprecipitation procedures
Add Trtion and Immunoprecipitation buffer to lyse cells and solubilize proteins.
Centrifuge debris and collect the supernatant
Add immunoprecipitating antibody to the supernatant
Add immunoprecipitating beads
Gently sediment and wash the immunoprecipitating beads
Elute the antibody-protein complex from the immunoprecipitating beads