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Immunoprecipitation Buffer w/ Triton X-100 (TIB-1)

Immunoprecipitation Buffer w/ Triton X-100 (TIB-1)

Manufacturer	Fivephoton
Category	Metabolism Obesity Reagents & Chemicals Biochemicals Buffers
Type	Buffer
Specific against	other
Amount	150 ml
Item no.	TIB-1
eClass 6.1	32129211
eClass 9.0	42051003
Available
Highlights	Applicable to immunoprecipitation-pull down procedures Maintains charge-charge and hydrogen bonding interactions between proteins, but abrogates hydrophobic interactions Efficient membrane dissolution capacity Can be used as a stand alone cell lysis buffer or in conjunction with downstream IP Formulated with a non-amine containing buffer that allows for minimal interference with protein concentration assays Can be used in cross-linking procedures
Background	The Triton Immunoprecipitation and Cell Lysis Buffer is applicable to most standard immunoprecipitation procedures. It is used to lyse cells, and solubilize proteins that can be co-immunoprecipitated. The lysis buffer abbrogates hydrophobic interactions but maintains charge-charge interactions between proteins. Synopsis of cell lysis and immunoprecipitation procedures Add Trtion and Immunoprecipitation buffer to lyse cells and solubilize proteins. Centrifuge debris and collect the supernatant Add immunoprecipitating antibody to the supernatant Add immunoprecipitating beads Gently sediment and wash the immunoprecipitating beads Elute the antibody-protein complex from the immunoprecipitating beads

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 150 ml

available

Delivery expected until 6/6/2024