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Mouse IL-1 beta ELISA Kit

ArtNr FPEK0394
Hersteller Fivephoton
Menge 96 T
Kategorie
Typ Elisa-Kit
Specific against Mouse (Murine, Mus musculus)
Citations 1. Takahashi, J. L.; Giuliani, F.; Power, C.; Imai, Y.; Yong, V. W. : Interleukin-1-beta promotes oligodendrocyte death through glutamate excitotoxicity. Ann. Neurol. 53: 588-595, 2003.

2. Baek, S. H.; Ohgi, K. A.; Rose, D. W.; Koo, E. H.; Glass, C. K.; Rosenfeld, M. G. : Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappa-B and beta-amyloid precursor protein. Cell 110: 55-67, 2002.

3. Voronov, E.; Shouval, D. S.; Krelin, Y.; Cagnano, E.; Benharroch, D.; Iwakura, Y.; Dinarello, C. A.; Apte, R. N. : IL-1 is required for tumor invasiveness and angiogenesis. Proc. Nat. Acad. Sci. 100: 2645-2650, 2003.

4. Chen, C.-N.; Li, Y.-S. J.; Yeh, Y.-T.; Lee, P.-L.; Usami, S.; Chien, S.; Chiu, J.-J. : Synergistic roles of platelet-derived growth factor-BB and interleukin-1-beta in phenotypic modulation of human aortic smooth muscle cells. Proc. Nat. Acad. Sci. 103: 2665-2670, 2006.
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Manufacturer - Targets
IL1B
Description
Highly sensitive kit to mouse IL-1 beta. Validated for sensitive and selective detection in cell culture medium, serum and plasma.
Background
This mouse IL-1 beta ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washes with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the concentration of IL-1 beta captured on the plate.
Highlights
Fully configured colorimetric ELISA kit in sandwich fomat.
Natural - purified protien provided as standard.
Assay range: 12.5pg/ml-800pg/ml.
Validated for cell culture medium, serum and plasma.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T
Lieferbar: In stock
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