Intracellular Immunofluorescence Labeling Kit (INF-1)

The Fivephoton Biochemicals intracellular immunofluorescence staining kit enables simultaneous labelling of multiple intracellular epitopes. The immunofluorescence kit uses bark saponin for membrane permeabilization, a reagent that permeates the outer cellular membrane as well as organeller membranes, and allows antibody to reach all cellular regions. Saponin used for membrane permeation faciliates high resoluion imaging since membraneous structures remain intact. Use primary antibodies that recognize intracellular domains to exclusively label intracellular epitopes, .

Immunofluorescence staining with membrane permeabilization is employed to visualize intracellular structures and organelles such as nuclei, actin filaments, Golgi, and endoplasmic reticulum. Intracellular cytosolic and organellar protein epitopes as well as intracellular domains of transmembrane proteins are labeled with this kit since antibodies have access to all intracellular regions.

(An important experimental distinction is provided in the Fivephoton Biochemicals Extracellular Immunofluorescence Kit (Part No. EIF-1) which enables exclusive extracellular labeling. The application in the Extracellular Immunofluorescence Labeling Kit does not include a membrane permeabilization reagent and therefore preserves membrane integrity).