Intracellular Immunofluorescence Labeling Kit (INF-1)

Manufacturer Fivephoton
Type Kit
Specific against other
Amount 1 kit
ArtNr NF-1-2
Eclass 6.1 32161000
Eclass 9.0 32161090
Poisonous Material
Sodium Azide (0.1%)
Immunofluorescence labeling with membrane permeabilization by using saponin.
Applicable for multiple-simultaneous immunofluorescent labeling as well as labeling of single epitopes.
Facilitates labeling of intracellular organelles (i.e. Golgi, mitochondria, endoplasmic reticulum, lysosomes and nuclei).
Stock solutions can be thawed and frozen multiple times, or aliquoted and frozen for later use.
Ideal for laboratory teaching as well as research applications.
The Fivephoton Biochemicals intracellular immunofluorescence staining kit enables simultaneous labelling of multiple intracellular epitopes. The immunofluorescence kit uses bark saponin for membrane permeabilization, a reagent that permeates the outer cellular membrane as well as organeller membranes, and allows antibody to reach all cellular regions. Saponin used for membrane permeation faciliates high resoluion imaging since membraneous structures remain intact. Use primary antibodies that recognize intracellular domains to exclusively label intracellular epitopes, .

Immunofluorescence staining with membrane permeabilization is employed to visualize intracellular structures and organelles such as nuclei, actin filaments, Golgi, and endoplasmic reticulum. Intracellular cytosolic and organellar protein epitopes as well as intracellular domains of transmembrane proteins are labeled with this kit since antibodies have access to all intracellular regions.

(An important experimental distinction is provided in the Fivephoton Biochemicals Extracellular Immunofluorescence Kit (Part No. EIF-1) which enables exclusive extracellular labeling. The application in the Extracellular Immunofluorescence Labeling Kit does not include a membrane permeabilization reagent and therefore preserves membrane integrity).
Blot V and McGraw TE. Use of quantitative immunofluorescence microscopy to study intracellular trafficking: studies of the GLUT4 glucose transporter. Methods Mol Biol 457: 347-366, 2008.

Constans J, Oksman F, and Viau M. Binding of the apo and holo forms of the serum vitamin D-binding protein to human lymphocyte cytoplasm and membrane by indirect immunofluorescence. Immunol Lett 3: 159-162, 1981.

Crivellato E, Travan L, Candussio L, Bartoli Klugmann F, and Decorti G. Identification of P-glycoprotein at the membrane of mast cell secretory granules. An immunofluorescence and protein A-gold electron microscopical investigation. Histochem J 29: 193-198, 1997.

Doody A and Putnam D. Identification of compartments involved in mammalian subcellular trafficking pathways by indirect immunofluorescence. Methods Mol Med 127: 127-136, 2006.

Fleischmajer R, Dessau W, Timpl R, Krieg T, Luderschmidt C, and Wiestner M. Immunofluorescence analysis of collagen, fibronectin, and basement membrane protein in scleroderma skin. J Invest Dermatol 75: 270-274, 1980.

Gajl-Peczalska K. Plasma Protein Composition Of Hyaline Membrane In The Newborn As Studies By Immunofluorescence. Arch Dis Child 39: 226-231, 1964.

Lejneva OM, Abelev GI, Dorfman NA, Strand M, and August JT. Localization of a murine oncornavirus 15, 000-dalton virion protein on the membrane of neoplastic cells: analysis by immunofluorescence and immunoelectron microscopy. Virology 75: 281-292, 1976.

Risau W, Saumweber H, and Symmons P. Monoclonal antibodies against a nuclear membrane protein of Drosophila. Localization by indirect immunofluorescence and detection of antigen using a new protein blotting procedure. Exp Cell Res 133: 47-54, 1981.

Tezuka T and Takahashi M. The 55-kd keratohyalin granule protein has the same epitope as the 43-kd stratum corneum membrane protein: immunofluorescence and immunoblotting studies using a monoclonal antibody to the 55-kd keratohyalin granule protein. Arch Dermatol Res 280: 462-468, 1989.

Wang H, Lee EW, Cai X, Ni Z, Zhou L, and Mao Q. Membrane topology of the human breast cancer resistance protein (BCRP/ABCG2) determined by epitope insertion and immunofluorescence. Biochemistry 47: 13778-13787, 2008.
Amount: 1 kit
Available: In stock
Listprice: €296.21
Price: €296.21

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