Intracellular Immunofluorescence Labeling Kit (INF-1)

Immunofluorescence labeling with membrane permeabilization by using saponin.
Applicable for multiple-simultaneous immunofluorescent labeling as well as labeling of single epitopes.
Facilitates labeling of intracellular organelles (i.e. Golgi, mitochondria, endoplasmic reticulum, lysosomes and nuclei).
Stock solutions can be thawed and frozen multiple times, or aliquoted and frozen for later use.
Ideal for laboratory teaching as well as research applications.

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Doody A and Putnam D. Identification of compartments involved in mammalian subcellular trafficking pathways by indirect immunofluorescence. Methods Mol Med 127: 127-136, 2006.

Fleischmajer R, Dessau W, Timpl R, Krieg T, Luderschmidt C, and Wiestner M. Immunofluorescence analysis of collagen, fibronectin, and basement membrane protein in scleroderma skin. J Invest Dermatol 75: 270-274, 1980.

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Risau W, Saumweber H, and Symmons P. Monoclonal antibodies against a nuclear membrane protein of Drosophila. Localization by indirect immunofluorescence and detection of antigen using a new protein blotting procedure. Exp Cell Res 133: 47-54, 1981.

Tezuka T and Takahashi M. The 55-kd keratohyalin granule protein has the same epitope as the 43-kd stratum corneum membrane protein: immunofluorescence and immunoblotting studies using a monoclonal antibody to the 55-kd keratohyalin granule protein. Arch Dermatol Res 280: 462-468, 1989.

Wang H, Lee EW, Cai X, Ni Z, Zhou L, and Mao Q. Membrane topology of the human breast cancer resistance protein (BCRP/ABCG2) determined by epitope insertion and immunofluorescence. Biochemistry 47: 13778-13787, 2008.