NF-kappa B (NF-KB) - p65 (RelA) Immunofluorescence Labeling Kit (NF-kB, NF-kappaB, NFKB-3)

The Cyto-MTM MTT Cell-Based Cytotoxicity Kit contains pre-configured reagents to rapidly quantitate cell number spectrophotometrically as a function of mitochondrial activity in living cells. This kit provides an alternative method to cell counting, and can be applied to most measurements of cell death or cell proliferation. The Cyto-MTM MTT Cell-based Toxicity Assay System kit yields accurate and reproducible data by using only three components. Furthermore, the kit was designed to create minimal disturbances to cells and limits detachment by not requiring frequent rinsing. The assay is also applicable in bacteria and fungus.

Cyto-MTM MTT Cell-based Toxicity Assay Kit Overrview
The key component of the assay is a proprietary concentration of MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide). MTT is a yellowish solution when dissolved in buffered salt solutions without phenol red and is taken up by cells due to its net positive charge. The tetrazolium ring of MTT (yellow) is reduced to purple formazan crystals by intracellular NAD(P)H-oxidoreductases. The resulting purple solution is spectrophotometrically measured.

Representative data of Cyto-MTM MTT Toxicity and Cell Proliferation Assay:
Figure Legend. The influence of cell number and cell types on absorbance at 570 nM. Various cell types were seeded in a 96 well dish at the density indicated in the figure. 24 hrs after seeding, the cells were processed using with the MTT assay kit. Absorbance was measured at 570 nm.