NF-kappa B (NF-KB) - p65 (RelA) Immunofluorescence Labeling Kit (NF-kB, NF-kappaB, NFKB-3)

Manufacturer Fivephoton
Category Products / Kits
Type Kit
Specific against other
Amount 1 kit
ArtNr NFKB-3
Eclass 6.1 32161000
Eclass 9.0 32161090
Available
Poisonous Material
Sodium Azide (0.1%)
Background
The Cyto-MTM MTT Cell-Based Cytotoxicity Kit contains pre-configured reagents to rapidly quantitate cell number spectrophotometrically as a function of mitochondrial activity in living cells. This kit provides an alternative method to cell counting, and can be applied to most measurements of cell death or cell proliferation. The Cyto-MTM MTT Cell-based Toxicity Assay System kit yields accurate and reproducible data by using only three components. Furthermore, the kit was designed to create minimal disturbances to cells and limits detachment by not requiring frequent rinsing. The assay is also applicable in bacteria and fungus.

Cyto-MTM MTT Cell-based Toxicity Assay Kit Overrview
The key component of the assay is a proprietary concentration of MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide). MTT is a yellowish solution when dissolved in buffered salt solutions without phenol red and is taken up by cells due to its net positive charge. The tetrazolium ring of MTT (yellow) is reduced to purple formazan crystals by intracellular NAD(P)H-oxidoreductases. The resulting purple solution is spectrophotometrically measured.

Representative data of Cyto-MTM MTT Toxicity and Cell Proliferation Assay:
Figure Legend. The influence of cell number and cell types on absorbance at 570 nM. Various cell types were seeded in a 96 well dish at the density indicated in the figure. 24 hrs after seeding, the cells were processed using with the MTT assay kit. Absorbance was measured at 570 nm.
References
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Cardin GB, Mantha M, Jumarie C. 2009. Resistance to cadmium as a function of Caco-2 cell differentiation: role of reactive oxygen species in cadmium- but not zinc-induced adaptation mechanisms. Biometals 22: 753-69.

Caruso M, Mariotti A, Zizzadoro C, Zaghini A, Ormas P, et al. 2009. A clonal cell line (BME-UV1) as a possible model to study bovine mammary epithelial metabolism: metabolism and cytotoxicity of aflatoxin B1. Toxicon 53: 400-8.

Cavalcanti BC, Bezerra DP, Magalhaes HI, Moraes MO, Lima MA, et al. 2009. Kauren-19-oic acid induces DNA damage followed by apoptosis in human leukemia cells. J Appl Toxicol 29: 560-8.

Cen L, Hutzen B, Ball S, DeAngelis S, Chen CL, et al. 2009. New structural analogues of curcumin exhibit potent growth suppressive activity in human colorectal carcinoma cells. BMC Cancer 9: 99

Chang MC, Lin LD, Chan CP, Chang HH, Chen LI, et al. 2009. The effect of BisGMA on cen species- and MEK/ERK-dependent and -independent pathways. Biomaterials 30: 4070-7.

Charupant K, Daikuhara N, Saito E, Amnuoypol S, Suwanborirux K, et al. 2009. Chemistry of renieramycins. Part 8: synthesis and cytotoxicity evaluation of renieramycin M-jorunnamycin A analogues. Bioorg Med Chem 17: 4548-58.
Amount: 1 kit
Available: In stock
Listprice: €508.71
Price: €508.71
lieferbar

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Instruction sheet

NFKB-3.pdf