Transmembrane Protein Extraction Reagent (tmPER-100)

The FIVEphoton Biochemicals Transmembrane Protein Extraction Reagent (tmPER-100TM) is a cell lysis-protein extraction buffer with proprietary ingredients assisting in the extraction and isolation of high molecular weight multiple-membrane spanning proteins that are otherwise poorly resolvable in standard cell lysis buffers due to aggregation tendency, lipid raft association or other insolubility issues. The Transmembrane Protein Extraction Reagent is designed to extract proteins with 4 or more transmembrane domains, yet will also effectively resolve less complex transmembrane proteins. The transmembrane protein extraction reagent is applicable to transmembrane proteins that do not appreciably solubilize or resolve with RIPA buffer.

Examples for the applicability of the FIVEphoton Biochemicals Transmembrane Protein Extraction Reagent include resolution of ABC transporters, ion channels, ion exchangers and GPCRs for the downstream application of Western blotting.