Transmembrane Protein Extraction Reagent (tmPER-100)

Manufacturer Fivephoton
Category Products / Proteins
Type Proteins
Specific against other
Amount 50 ml
ArtNr tmPER-100
Eclass 6.1 34160400
Eclass 9.0 42020190
Available
Highlights
Significantly higher solubilzation and resolving ability compared to RIPA buffer for large transmembrane proteins.
Facilitates resolution of high MW transmembrane proteins in Western blots.
Ideal for multi-pass (4+ transmembrane domains) proteins that aggregate.
Contains a proprietary ingredient to extract TM proteins embedded in lipid rafts.
pH 7.4. 1X solution. 50 ml volume.
Compatible with BCA assay.
Storage: 4C. Ships at ambient temperature
Background
The FIVEphoton Biochemicals Transmembrane Protein Extraction Reagent (tmPER-100TM) is a cell lysis-protein extraction buffer with proprietary ingredients assisting in the extraction and isolation of high molecular weight multiple-membrane spanning proteins that are otherwise poorly resolvable in standard cell lysis buffers due to aggregation tendency, lipid raft association or other insolubility issues. The Transmembrane Protein Extraction Reagent is designed to extract proteins with 4 or more transmembrane domains, yet will also effectively resolve less complex transmembrane proteins. The transmembrane protein extraction reagent is applicable to transmembrane proteins that do not appreciably solubilize or resolve with RIPA buffer.

Examples for the applicability of the FIVEphoton Biochemicals Transmembrane Protein Extraction Reagent include resolution of ABC transporters, ion channels, ion exchangers and GPCRs for the downstream application of Western blotting.
References
Berberian G, Bollo M, Montich G, Roberts G, Degiorgis JA, et al. 2009. A novel lipid binding protein is a factor required for MgATP stimulation of the squid nerve Na+/Ca2+ exchanger. Biochim Biophys Acta 1788: 1255-62.

Castro-Parodi M, Levi L, Dietrich V, Zotta E, Damiano AE. 2009. CFTR may modulate AQP9 functionality in preeclamptic placentas. Placenta 30: 642-8.

Chen WY, Xu WM, Chen ZH, Ni Y, Yuan YY, et al. 2009. Cl- is required for HCO3- entry necessary for sperm capacitation in guinea pig: involvement of a Cl-/HCO3- exchanger (SLC26A3) and CFTR. Biol Reprod 80: 115-23.

Gad A, Callender DL, Killeen E, Hudak J, Dlugosz MA, et al. 2009. Transient in utero disruption of cystic fibrosis transmembrane conductance regulator causes phenotypic changes in alveolar type II cells in adult rats. BMC Cell Biol 10: 24.
Amount: 50 ml
Available: In stock
Listprice: €133.41
Price: €133.41
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