Chk1 Antibody (Phospho-Ser296), pAb, Rabbit

USA

GenScript Rabbit Anti-Chk1 (Phospho-Ser296) Polyclonal Antibody detects endogenous levels of Chk1 only when phosphorylated at serine 296.

Chk1 is a serine/threonine kinase that was first identified as a checkpoint kinase in, Schizosaccharomyces pombe . Chk1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. Chk1 activated through phosphorylation by ATR/ATM and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression. The activation of Chk1 in response to blocking DNA replication and certain forms of genotoxic stress involves phosphorylation of Ser 317 and Ser 345. Chk1 is also phosphorylated at Ser 280 and Ser 296 following DNA damage. Activated Chk1 phosphorylates downstream factors that mediate cell cycle arrest and other physiological events in the cell, these key cell cycle factors include p53, Cdc25C, etc. GenScript, Rabbit Anti-Chk1 (Phospho-Ser296) Polyclonal Antibody, is developed in rabbit using a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser 296 of human Chk1.

GenScript Rabbit Anti-Chk1 (Phospho-Ser296) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.