Fibroblast Growth Factor-basic ( FGF-basic ), Bovine

Hersteller GenScript
Kategorie
Typ Proteins
Specific against Bovine
Menge 50ug 
ArtNr Z02789-50
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Product Line
Cytokine, Chemokines & Growth Factors
Description
bFGF is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the FGF-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor.
M.W.
Approximately 16.4 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids
Purity
>95% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rBoFGF-2 as determined by LAL method.
Specific Activity
Fully biologically active when compared to standard. The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 cells is < 1.0ng/ml, corresponding to a specific activity of 1.0 x 106 Units/mg.
Storage
This lyophilized preparation is stable for several weeks at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4, containing 4% mannitol.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
MTMITNSSSV PGDPLESMAA GSITTLPALP EDGGSGAFPP GHFKDPKRLYCKNGGFFLRI HPDGRVDGVR EKSDPHIKLQ LQAEERGVVS IKGVCANRYLAMKEDGRLLA SKCVTDECFF FERLESNNYN TYRSRKYSSW YVALKRTGQYKLGPKTGPGQ KAILFLPMSA KS
Menge: 50ug 
Lieferbar: Out of stock
Listenpreis: 227,50 €
Preis: 227,50 €
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