Hersteller |
GenScript
|
Kategorie |
|
Typ |
Proteins |
Specific against |
Human |
Menge |
1 mg |
ArtNr |
Z02022-1 |
Targets |
RTEL1, CXCL8, CD154, RND1, IFNG, CD24, TNF |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Specificity |
Recombinant human CD40 Ligand (CD40L/CD154/TRAP), is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg. |
Description |
CD40 Ligand (CD40L/CD154/TRAP), is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Recombinant Human CD40 Ligand (CD40L/CD154/TRAP), produced in, E. coli, is a non-glycosylated, polypeptide chain containing 149 amino acids and having a molecular mass of 16, 308 Da. |
Endotoxin Level |
Less than 0.1 ng/ug (1 EU/ug) of recombinant human CD40 Ligand (CD40L) as determined by LAL test. |
Formulation |
The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH8.0, 250mM NaCl buffer. |
M.W. |
16, 308 Da |
Product Line |
Cytokine, Chemokines & Growth Factors |
Reconstitution |
It is recommended to reconstitute the lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Storage |
Lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CD40L should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Manufacturers Category |
Proteins |
Manufacturers Product Line |
Immune Checkpoints |
Product Origin |
USA |
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