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SCF, Human (P. pastoris-expressed)

SCF, Human (P. pastoris-expressed)

ArtNr	Z02692-1
Hersteller	GenScript
Menge	1 mg
Quantity options	1 mg 10 ug 50 ug
Kategorie	Oncology Peptides & Proteins Recombinant Proteins By Organ Lung Cancer Bone Cancer
Typ	Proteins
Specific against	Human (Homo sapiens)
Purity	> 95% by SDS-PAGE and HPLC analyses.
Sequence	Glu26-Ala189 (accession number: P21583.1), expressed with an N-terminal Met.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02692-Stem_Cell_Factor_SCF_human,Stem Cell Factor (SCF), is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human Stem Cell Factor (rhSCF), produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ca.20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> ca.20 kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <1.0 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5×105 IU/mg. </td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human Stem Cell Factor (rhSCF), remains stable up to 12 months at -80C from date of receipt. Upon reconstitution, rhSCF should be stable up to 4 weeks at 4C or up to 6 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against 10 mM acetic acid.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Glu26-Ala189 (accession number: P21583.1), expressed with an N-terminal Met.</td></tr>
Similar products	Stem
Versandbedingung	Gekühlt
Lieferbar
Specificity	The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5x105 IU/mg.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	ca.20 kDa, observed by reducing SDS-PAGE.
Product Line	Growth Factors
Manufacturer - Specificity	The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5x105 IU/mg.
Description	Stem Cell Factor (SCF), is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human Stem Cell Factor (rhSCF), produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ca.20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against 10 mM acetic acid.
Endotoxin Level	<1.0 EU/ug, determined by LAL method.

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Menge: 1 mg

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