|
Hersteller |
GenScript
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
10.0ug |
|
ArtNr |
Z02714-10 |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Product Line |
Cytokine, Chemokines & Growth Factors |
| Description |
IL-20 is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences but they are dissimilar in their biological functions. IL-20 is a hematopoietic growth factor capable of stimulating colony formation by CD34+ multipotential progenitors, but not by other progenitor cells. IL-20 signals through a receptor system composed of type I IL-20R-ª and type II IL-20R-ß. Over-expression of IL-20 in keratinocytes expressing both receptor subunits has been implicated in the induction of inflammatory skin disease. |
| M.W. |
Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Purity |
>95% by SDS-PAGE and HPLC analyses. |
| Endotoxin Level |
Less than 0.2EU/ug of rHuIL-20 as determined by LAL method. |
| Specific Activity |
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using human IL-20Rα, and human IL-20Rβ, co-transfected murine BaF3 pro-B cells is less than 0.6 ng/ml, corresponding to a specific activity of ＞, 1.7 x 106 IU/mg. |
| Storage |
This lyophilized preparation is stable for several weeks at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles. |
| Formulation |
Lyophilized from a 0.2µ, m filtered concentrated, solution in PBS, pH 7.4. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °, C. Further dilutions should be made in appropriate buffered solutions. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Usage |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Sequence |
MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQDTKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDLRLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE |
