|
Hersteller |
GenScript |
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
10ug |
|
ArtNr |
Z02717-10 |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Product Line |
Cytokine, Chemokines & Growth Factors |
| Description |
Human IL-31 is a T-cell derived cytokine that shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. It signals through a receptor complex comprised of GPL (GP130-like, IL-31RA) and OSMR (Oncostatin M receptor). GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. |
| M.W. |
Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids. |
| Purity |
>97% by SDS-PAGE and HPLC analyses. |
| Endotoxin Level |
Less than 0.2EU/ug of rHuIL-31 as determined by LAL method. |
| Specific Activity |
Fully biologically active when compared to standard.Measured by its ability to induce STAT3 activation in U-87 MG human glioblastoma/astrocytoma cells. 5 ng/mL of rhIL31 can effectively induce STAT3 activation. |
| Storage |
This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles. |
| Formulation |
Lyophilized from a 0.2µ, m filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °, C. Further dilutions should be made in appropriate buffered solutions. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Usage |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Sequence |
SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T |
