|
Hersteller |
GenScript |
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
1mg |
|
ArtNr |
Z02754-1 |
|
Eclass 6.1 |
34160400 |
|
Eclass 9.0 |
42020190 |
|
Lieferbar |
 |
| Product Line |
Cytokine, Chemokines & Growth Factors |
| Description |
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The ª-defensins are distinguished from the ß-defensins by the pairing of their three disulfide bonds. To date, four human ß-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. ß-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The ß-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. ß-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. ß-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. |
| M.W. |
Approximately 5.0 KDa, a single non-glycosylated polypeptide chain containing 47 amino acids. |
| Purity |
>98% by SDS-PAGE and HPLC analyses. |
| Endotoxin Level |
Less than 0.2EU/ug of rHuBD-1 as determined by LAL method. |
| Specific Activity |
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay usingCD34+ dendritic cells, is less than 1000 ng/ml, corresponding to a specific activity of ＞, 1.0 x 103 IU/mg. |
| Storage |
This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles. |
| Formulation |
Lyophilized from a 0.2µ, m filtered concentrated, solution in 20mM PB, pH 7.4, 130mM NaCl. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °, C. Further dilutions should be made in appropriate buffered solutions. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Usage |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Sequence |
GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK |
