beta-Defensin 4 ( BD-4 ), Human

Hersteller GenScript
Kategorie
Typ Proteins
Specific against Human
Menge 1mg 
ArtNr Z02757-1
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Product Line
Cytokine, Chemokines & Growth Factors
Description
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The ª-defensins are distinguished from the ß-defensins by the pairing of their three disulfide bonds. To date, four human ß-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. The ß-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. ß-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. ß-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes.
M.W.
Approximately 6.0 KDa, a single non-glycosylated polypeptide chain containing 50 amino acids.
Purity
>98% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rHuBD-4 as determined by LAL method.
Specific Activity
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay usinghuman monocytes is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 x 104 IU/mg.
Storage
This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2µ, m filtered concentrated, solution in 20mM PB, pH 7.4, 130mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°, C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Sequence
EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP
Menge: 1mg 
Lieferbar: In stock
Listenpreis: 2.695,55 €
Preis: 2.695,55 €
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